Arsene Wengers side play the star-studded Spanish giants, in what is a repeat of the 2006 final, which the Spanish champions won 2-1 thanks to two late goals in Paris.
Jose Mourinho’s Chelsea will face Paris Saint-Germain for the second year running, after losing to the French giants last season.
Italian side Roma play Real Madrid, while last year’s finalist Juventus, were drawn against German Champions, Bayern Munich.
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Also, Manchester City play Ukrainian side, Dynamo Kiev.
First legs are scheduled to take place on 16-17 and 23-24 February, while the return legs are scheduled to take place on 8-9 and 15-16 March.
The Draw in full:
Gent v Wolfsburg
Roma v Real Madrid
Paris St-Germain v Chelsea
Arsenal v Barcelona
Juventus v Bayern Munich
PSV Eindhoven v Atletico Madrid
Benfica v Zenit St Petersburg
Dynamo Kiev v Manchester City